# Ph and buffer relationship help

### pH and pKa relationship for buffers (video) | Khan Academy

pH and pKa Relationship: The Henderson-Hasselbalch Equation specific, in that it helps you predict what a molecule will do at a specific pH. −1 buffers should be x greater than that of. The kidneys and the lungs work together to help maintain a blood pH of by . However, the relationship shown in Equation 11 is frequently referred to as the. Answer to What is the relationship between buffer and pH stability? Buffer is a solution that resist change in the pH of a solution. Generally they are made of combination of help from Chegg. Get help now from expert Biology tutors.

So let's go ahead and look at all the possible scenarios for these three things.

So anything to the zeroth power is equal to one. Which tells us that this ratio is equal to one.

### pH Buffers in the Blood

And if A minus concentration over HA concentration is equal to one, that means that they have the same concentration. I forgot a minus sign there. This is a really helpful thing to remember.

And this comes up a lot not just when you're talking about buffers by themselves, but also when you're doing titrations. And the point in your titration where the HA is equal to A minus is called the half-equivalence point. And if you haven't learnt about buffers, that's okay. Oh, sorry, if you haven't learn about titrations yet, that's fully fine.

Just ignore what I just said laughsbut if you have, the moral is just that, this is a really, really important relationship that is really helpful to remember. And I said really a lot there.

And when you raise 10 to a positive number, when you raise 10 to a positive number, you get a ratio that is greater than one. So if our ratio A minus over HA is greater than one, that tells us that A minus, the numerator, is actually greater than the denominator, HA.

And that means that our ratio, A minus over HA, is actually less than one. So that tells us that our denominator, HA, is actually bigger than our numerator. It's very easy to derive. We did it in just a few minutes, so it's okay if you don't remember this all the time. Many people today are interested in exercise as a way of improving their health and physical abilities. But there is also concern that too much exercise, or exercise that is not appropriate for certain individuals, may actually do more harm than good.

Exercise has many short-term acute and long-term effects that the body must be capable of handling for the exercise to be beneficial. Some of the major acute effects of exercising are shown in Figure 1.

When we exercise, our heart rate, systolic blood pressure, and cardiac output the amount of blood pumped per heart beat all increase.

Blood flow to the heart, the muscles, and the skin increase.

## How can we predict the pH of a buffer?

We breathe faster and deeper to supply the oxygen required by this increased metabolism. Eventually, with strenuous exercise, our body's metabolism exceeds the oxygen supply and begins to use alternate biochemical processes that do not require oxygen. These processes generate lactic acid, which enters the blood stream. As we develop a long-term habit of exercise, our cardiac output and lung capacity increase, even when we are at rest, so that we can exercise longer and harder than before.

Over time, the amount of muscle in the body increases, and fat is burned as its energy is needed to help fuel the body's increased metabolism. Figure 1 This figure highlights some of the major acute short-term effects on the body during exercise. Dialysis in the Kidneys " you learned about the daily maintenance required in the blood for normal everyday activities such as eating, sleeping, and studying.

Now, we turn our attention to the chemical and physiological concepts that explain how the body copes with the stress of exercise. As we shall see, many of the same processes that work to maintain the blood's chemistry under normal conditions are involved in blood-chemistry maintenance during exercise, as well.

During exercise, the muscles use up oxygen as they convert chemical energy in glucose to mechanical energy. This O2 comes from hemoglobin in the blood. These chemical changes, unless offset by other physiological functions, cause the pH of the blood to drop. If the pH of the body gets too low below 7. This can be very serious, because many of the chemical reactions that occur in the body, especially those involving proteins, are pH-dependent.

Ideally, the pH of the blood should be maintained at 7. If the pH drops below 6. Fortunately, we have buffers in the blood to protect against large changes in pH. This external fluid, in turn, exchanges chemicals with the blood being pumped throughout the body. A dominant mode of exchange between these fluids cellular fluid, external fluid, and blood is diffusion through membrane channels, due to a concentration gradient associated with the contents of the fluids.

Recall your experience with concentration gradients in the "Membranes, Proteins, and Dialysis" experiment.

### Predicting the pH of a Buffer

Hence, the chemical composition of the blood and therefore of the external fluid is extremely important for the cell. As mentioned above, maintaining the proper pH is critical for the chemical reactions that occur in the body. In order to maintain the proper chemical composition inside the cells, the chemical composition of the fluids outside the cells must be kept relatively constant.

This constancy is known in biology as homeostasis. Figure 2 This is a schematic diagram showing the flow of species across membranes between the cells, the extracellular fluid, and the blood in the capillaries. The body has a wide array of mechanisms to maintain homeostasis in the blood and extracellular fluid. The most important way that the pH of the blood is kept relatively constant is by buffers dissolved in the blood. Other organs help enhance the homeostatic function of the buffers.

The kidneys help remove excess chemicals from the blood, as discussed in the Kidney Dialysis tutorial. Acidosis that results from failure of the kidneys to perform this excretory function is known as metabolic acidosis.

However, excretion by the kidneys is a relatively slow process, and may take too long to prevent acute acidosis resulting from a sudden decrease in pH e. The lungs provide a faster way to help control the pH of the blood. The increased-breathing response to exercise helps to counteract the pH-lowering effects of exercise by removing CO2, a component of the principal pH buffer in the blood.

Acidosis that results from failure of the lungs to eliminate CO2 as fast as it is produced is known as respiratory acidosis. A Quantitative View The kidneys and the lungs work together to help maintain a blood pH of 7. Therefore, to understand how these organs help control the pH of the blood, we must first discuss how buffers work in solution.

Acid-base buffers confer resistance to a change in the pH of a solution when hydrogen ions protons or hydroxide ions are added or removed. An acid-base buffer typically consists of a weak acid, and its conjugate base salt see Equations in the blue box, below.

pH and Buffers

Buffers work because the concentrations of the weak acid and its salt are large compared to the amount of protons or hydroxide ions added or removed. When protons are added to the solution from an external source, some of the base component of the buffer is converted to the weak-acid component thus using up most of the protons added ; when hydroxide ions are added to the solution or, equivalently, protons are removed from the solution; see Equations in the blue box, belowprotons are dissociated from some of the weak-acid molecules of the buffer, converting them to the base of the buffer and thus replenishing most of the protons removed.

However, the change in acid and base concentrations is small relative to the amounts of these species present in solution. Hence, the ratio of acid to base changes only slightly.

The Carbonic-Acid-Bicarbonate Buffer in the Blood By far the most important buffer for maintaining acid-base balance in the blood is the carbonic-acid-bicarbonate buffer. The simultaneous equilibrium reactions of interest are. Hence, the conjugate base of an acid is the species formed after the acid loses a proton; the base can then gain another proton to return to the acid.

In solution, these two species the acid and its conjugate base exist in equilibrium. Recall from this and earlier experiments in Chem and the definition of pH: When an acid is placed in water, free protons are generated according to the general reaction shown in Equation 3.